The study was conducted at Bangladesh Forest Research Institute, Chattogram to assess the effect of different growing media on germination and growth of Dipterocarpus turbinatus and Artocarpus chama seedlings in the nursery. The media used in the experiment were T1: Soil and Cow dung (3:1), T2 : Cocomoss/ processed coir dust and T3: Processed saw dust. Experiment was conducted by using Completely Randomized Design (CRD) with five replications of each three media. Germination percentage, survival percentage and growth performance of both species were recorded and analyzed. The result showed that the highest germination percentage (78%) of Dipterocarpus turbinatus in T3 and highest survival % in T1 while T2 had shown the highest rate of height (28.5cm), root collar diameter (3.8 mm), leaf number (10) and root length (30.2cm) of seedlings of D. turbinatus. On the other hand, highest rate of germination percentage (82%), survival percentage (78%), height (32.5cm), root collar diameter (4.52 mm), leaf number (9.0) and root length (29.7cm) for seedlings of A. chama also found in T2 media than two others media T1 and T3. Both species showed the highest growth performance in T2. The findings also explored T2 as a good quality co supplement and substitute of T1 media for raising the seedlings of selected species in the nursery.