An efficient in vitro protocol was established for large production of Aloe vera (Aloe indica Royle). The shoot tip explants, collected from the axenic cultures were used for the optimization of rapid shoot production on MS medium supplemented with different concentrations (0.5, 1.0, 2.0 and 3.0 mg/L) of BAP and Kn. The maximum shoot number were recorded as 14.33 per culture on medium added with MS + 1.0 mg/L BAP + 4% sucrose + 2.8 g/L gelrite after 30 days of culture. The shoots were rooted under in vitro and in vivo condition. About 90% shoots produced root on medium, Vi MS + 0.5 mg/L IBA+ 2% sugar after 30 days of culture. Simultaneously in vitro grown shoots were inoculated in the sand made propagation bed for ex vitro rooting under mist house condition. In the propagation bed 100% of the micro shoots survived and well rooted while taking a longer time (8 weeks) than that of in vitro rooting. The rooted seedlings were transferred in polybag containing garden soil, compost and sand with the proportion of 1:1:1 respectively. After hardening 99% seedlings survived in polybag and showed excellent growth.