A Half-yearly Peer Reviewed Journal of Bangladesh Forest Research Institute

ISSN - Print: 1021-3279 | Online: -

Dendrocalamus giganteus is one of the biggest and largest bamboo of
Bangladesh which is locally known as bhudum bansh. The conventional
propagation methods of bamboos are not economically viable for large scale
production due to their scarcity of seeds, low multiplication rate, labor intensive
and high cost. In vitro propagation is becoming a promising tool for conserving
and mass propagation of different bamboo species. In this study establishment
of a reliable and reproducible protocol for the micro propagation of D.
giganteus from axenic culture of in vitro germinated seedlings has been
reported. Highest 83.33% seeds were germinated on MS (Murashige and
Skooge 1962) medium supplemented with 1.0 mg/L BAP (6-benzyl
-amino-purine) after 7 days of culture. MS supplemented with different
concentrations (0.0, 1.0, 2.0, 3.0 and 4.0 mg/L) of BAP and Kn
(6-furfuralaminopurine) at evaluated either singly or in combinations for
multiple shoot production. Maximum 16.33 numbers of young shoots per
culture were recorded in medium supplemented with MS + 3.0 mg/L BAP +
1.0 mg/L Kn + 4% sugar + 2.75 g/L after 28 days of culture. Rooting ability of
the shoots was assessed in half strength MS media supplemented with different
concentrations (0.0, 1.0, 2.0, 3.0, and 4.0 mg/L) of IBA (Indole-3-butyric acid).
The highest rooting percentage (66.67%) was achieved from the half strength
MS medium supplemented with 1.0 mg/L IBA 2 after weeks of culture. The
rooted plantlets were successfully hardened in soil under greenhouse and
nursery of Silviculture Genetics Division, Bangladesh Forest Research
Institute. The survival percentage of tissue culture plantlets in nursery was
found to be 90-95% after 60 days of acclimatization. The protocol developed
through this study enable to produce large number of D. giganteus bamboo
seedlings for mass propagation in a short period of time.


Dendrocalamus hamiltonii and Schizostachyum dullooa are the two
important threatened bamboo species of Bangladesh. They flowered gregariously during 1996 to 1998 and 1999 at the bambusetum of Bangladesh Forest
Research Institute,Chittagong and RangapaniTeaEstate, Hazarikhil, Chittagong
respectively. The flowering cyclesfor D. hamiltonii were estimated as 43 + 5 years
and for S. dullooa as 45 + 2, 35 + 2 and 15 + 2 years. Observations were made on
flowering nature, death and seed characters of these species. The germination
and seedling characters were also recorded. Planta tions of both the species were
raised with seedlings by the Forest Department and the NGOs. In future these
will be used as ex situ conservation plots.


Seed germination remains to be an unsolved problem in teak. Studies made on
radiography and seed technological aspects using drupes from India and Thailand
disclosed certain important features. Radiography has been standardised for sharp
image to read the anatomical potential of seed. Teak drupes from two provenance
differed in size and weight within and between seedlots. Radiograms revealed the
development of 4, 3,2 and 1 seed in the tetracarpellary ovary in the order of 1,5,19
and 48% with 27% empty drupes. When drupe size decreased the percentage of
single-seeded and empty drupes increased correspondingly. Teak drupes possess
ontogenic and dormancy problems posing challenge to germination


This paper discusses the effect of relative humidity during storage on seed
longevity and sowing depth on germination of Prosopsis africana (Guill and Perr.)
Taub seeds. Freshly collected and two-years old seeds were used for this experiment.
Six levels of relative humidity (RH) values ranging from 22 and 100% were
obtained by using water and saturated solutions of some chemicals in closed
chambers at a temperature of 28 – 30°C. Sundrying did not change the initial
moisture contents of fresh seed, while RH of 0 – 22% and 72.5 – 100% degraded the
seeds resulting in a rapid loss of viability. Complete loss of viability occurred within
18-21 months at low RH regimes and 9-15 months at high RH regimes. Germination
and emergence of seedlings were significantly favoured when seeds were sown at
a sowing depth range of 2.6 cm.

Last Published

Welcome to the Bangladesh Journal of Forest Science (BJFS) – a leading platform for advancing the knowledge and understanding of forest science in Bangladesh and beyond. Established with a commitment to excellence, BJFS serves as a cornerstone for researchers, practitioners, and enthusiasts dedicated to the sustainable management and conservation of forest ecosystems.