BANGLADESH JOURNAL OF FOREST SCIENCE
A Half-yearly Peer Reviewed Journal of Bangladesh Forest Research Institute
A Half-yearly Peer Reviewed Journal of Bangladesh Forest Research Institute
Dendrocalamus giganteus is one of the biggest and largest bamboo of Bangladesh which is locally known as bhudum bansh. The conventional
propagation methods of bamboos are not economically viable for large scale production due to their scarcity of seeds, low multiplication rate, labor intensive and high cost. In vitro propagation is becoming a promising tool for conserving and mass propagation of different bamboo species. In this study establishment of a reliable and reproducible protocol for the micro propagation of D. giganteus from axenic culture of in vitro germinated seedlings has been reported. Highest 83.33% seeds were germinated on MS (Murashige and Skooge 1962) medium supplemented with 1.0 mg/L BAP (6-benzyl -amino-purine) after 7 days of culture. MS supplemented with different concentrations (0.0, 1.0, 2.0, 3.0 and 4.0 mg/L) of BAP and Kn (6-furfuralaminopurine) at evaluated either singly or in combinations for multiple shoot production. Maximum 16.33 numbers of young shoots per culture were recorded in medium supplemented with MS + 3.0 mg/L BAP + 1.0 mg/L Kn + 4% sugar + 2.75 g/L after 28 days of culture. Rooting ability of the shoots was assessed in half strength MS media supplemented with different concentrations (0.0, 1.0, 2.0, 3.0, and 4.0 mg/L) of IBA (Indole-3-butyric acid). The highest rooting percentage (66.67%) was achieved from the half strength MS medium supplemented with 1.0 mg/L IBA 2 after weeks of culture. The rooted plantlets were successfully hardened in soil under greenhouse and nursery of Silviculture Genetics Division, Bangladesh Forest Research Institute. The survival percentage of tissue culture plantlets in nursery was found to be 90-95% after 60 days of acclimatization. The protocol developed through this study enable to produce large number of D. giganteus bamboo seedlings for mass propagation in a short period of time.
Dendrocalamus hamiltonii and Schizostachyum dullooa are the two important threatened bamboo species of Bangladesh. They flowered gregariously during 1996 to 1998 and 1999 at the bambusetum of Bangladesh Forest Research Institute,Chittagong and RangapaniTeaEstate, Hazarikhil, Chittagong respectively. The flowering cyclesfor D. hamiltonii were estimated as 43 + 5 years and for S. dullooa as 45 + 2, 35 + 2 and 15 + 2 years. Observations were made on flowering nature, death and seed characters of these species. The germination and seedling characters were also recorded. Planta tions of both the species were raised with seedlings by the Forest Department and the NGOs. In future these will be used as ex situ conservation plots.
Seed germination remains to be an unsolved problem in teak. Studies made on radiography and seed technological aspects using drupes from India and Thailand disclosed certain important features. Radiography has been standardised for sharp image to read the anatomical potential of seed. Teak drupes from two provenance differed in size and weight within and between seedlots. Radiograms revealed the development of 4, 3,2 and 1 seed in the tetracarpellary ovary in the order of 1,5,19 and 48% with 27% empty drupes. When drupe size decreased the percentage of
single-seeded and empty drupes increased correspondingly. Teak drupes possess ontogenic and dormancy problems posing challenge to germination
This paper discusses the effect of relative humidity during storage on seed longevity and sowing depth on germination of Prosopsis africana (Guill and Perr.) Taub seeds. Freshly collected and two-years old seeds were used for this experiment. Six levels of relative humidity (RH) values ranging from 22 and 100% were obtained by using water and saturated solutions of some chemicals in closed chambers at a temperature of 28 – 30°C. Sundrying did not change the initial moisture contents of fresh seed, while RH of 0 – 22% and 72.5 – 100% degraded the seeds resulting in a rapid loss of viability. Complete loss of viability occurred within 18-21 months at low RH regimes and 9-15 months at high RH regimes. Germination and emergence of seedlings were significantly favoured when seeds were sown at a sowing depth range of 2.6 cm.
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