BANGLADESH JOURNAL OF FOREST SCIENCE
A Half-yearly Peer Reviewed Journal of Bangladesh Forest Research Institute
A Half-yearly Peer Reviewed Journal of Bangladesh Forest Research Institute
An efficient in vitro protocol was established for large production of Aloe vera (Aloe indica Royle). The shoot tip explants, collected from the axenic cultures were used for the optimization of rapid shoot production on MS medium supplemented with different concentrations (0.5, 1.0, 2.0 and 3.0 mg/L) of BAP and Kn. The maximum shoot number were recorded as 14.33 per culture on medium added with MS + 1.0 mg/L BAP + 4% sucrose + 2.8 g/L gelrite after 30 days of culture. The shoots were rooted under in vitro and in vivo condition. About 90% shoots produced root on medium, Vi MS + 0.5 mg/L IBA+ 2% sugar after 30 days of culture. Simultaneously in vitro grown shoots were inoculated in the sand made propagation bed for ex vitro rooting under mist house condition. In the propagation bed 100% of the micro shoots survived and well rooted while taking a longer time (8 weeks) than that of in vitro rooting. The rooted seedlings were transferred in polybag containing garden soil, compost and sand with the proportion of 1:1:1 respectively. After hardening 99% seedlings survived in polybag and showed excellent growth.
Dendrocalamus giganteus is one of the biggest and largest bamboo of Bangladesh which is locally known as bhudum bansh. The conventional
propagation methods of bamboos are not economically viable for large scale production due to their scarcity of seeds, low multiplication rate, labor intensive and high cost. In vitro propagation is becoming a promising tool for conserving and mass propagation of different bamboo species. In this study establishment of a reliable and reproducible protocol for the micro propagation of D. giganteus from axenic culture of in vitro germinated seedlings has been reported. Highest 83.33% seeds were germinated on MS (Murashige and Skooge 1962) medium supplemented with 1.0 mg/L BAP (6-benzyl -amino-purine) after 7 days of culture. MS supplemented with different concentrations (0.0, 1.0, 2.0, 3.0 and 4.0 mg/L) of BAP and Kn (6-furfuralaminopurine) at evaluated either singly or in combinations for multiple shoot production. Maximum 16.33 numbers of young shoots per culture were recorded in medium supplemented with MS + 3.0 mg/L BAP + 1.0 mg/L Kn + 4% sugar + 2.75 g/L after 28 days of culture. Rooting ability of the shoots was assessed in half strength MS media supplemented with different concentrations (0.0, 1.0, 2.0, 3.0, and 4.0 mg/L) of IBA (Indole-3-butyric acid). The highest rooting percentage (66.67%) was achieved from the half strength MS medium supplemented with 1.0 mg/L IBA 2 after weeks of culture. The rooted plantlets were successfully hardened in soil under greenhouse and nursery of Silviculture Genetics Division, Bangladesh Forest Research Institute. The survival percentage of tissue culture plantlets in nursery was found to be 90-95% after 60 days of acclimatization. The protocol developed through this study enable to produce large number of D. giganteus bamboo seedlings for mass propagation in a short period of time.
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