A Half-yearly Peer Reviewed Journal of Bangladesh Forest Research Institute

ISSN - Print: 1021-3279 | Online: -

Dendrocalamus giganteus is one of the biggest and largest bamboo of
Bangladesh which is locally known as bhudum bansh. The conventional
propagation methods of bamboos are not economically viable for large scale
production due to their scarcity of seeds, low multiplication rate, labor intensive
and high cost. In vitro propagation is becoming a promising tool for conserving
and mass propagation of different bamboo species. In this study establishment
of a reliable and reproducible protocol for the micro propagation of D.
giganteus from axenic culture of in vitro germinated seedlings has been
reported. Highest 83.33% seeds were germinated on MS (Murashige and
Skooge 1962) medium supplemented with 1.0 mg/L BAP (6-benzyl
-amino-purine) after 7 days of culture. MS supplemented with different
concentrations (0.0, 1.0, 2.0, 3.0 and 4.0 mg/L) of BAP and Kn
(6-furfuralaminopurine) at evaluated either singly or in combinations for
multiple shoot production. Maximum 16.33 numbers of young shoots per
culture were recorded in medium supplemented with MS + 3.0 mg/L BAP +
1.0 mg/L Kn + 4% sugar + 2.75 g/L after 28 days of culture. Rooting ability of
the shoots was assessed in half strength MS media supplemented with different
concentrations (0.0, 1.0, 2.0, 3.0, and 4.0 mg/L) of IBA (Indole-3-butyric acid).
The highest rooting percentage (66.67%) was achieved from the half strength
MS medium supplemented with 1.0 mg/L IBA 2 after weeks of culture. The
rooted plantlets were successfully hardened in soil under greenhouse and
nursery of Silviculture Genetics Division, Bangladesh Forest Research
Institute. The survival percentage of tissue culture plantlets in nursery was
found to be 90-95% after 60 days of acclimatization. The protocol developed
through this study enable to produce large number of D. giganteus bamboo
seedlings for mass propagation in a short period of time.


Dendrocalamus giganteus, known as giant
bamboo, is the tallest among the bamboos,
measuring on an average about 25 m high and 25
cm diameter. It is cultivated in Cox’s Bazar,
Banderban, Chittagong, Mymensingh, Panchagar,
Jessore and some other Districts ofBangladesh. Its
local names are wara bans, bhudumbans, bombai
bans, raja bans, kanchan bans, etc. It grows
luxuriantly in all the flood free areas.
It was reported earlier that D. giganteus
flowered at Calcutta Botanical Garden, India in
1860-61 and again in 1888 and in Burma in 1892
(Gamble 1896). Lahiri (1974) reported that one
clump introduced during 1880-88 at Kurseong
Division, India flowered in 1974. Bahadur (1979)
reported flowering of this species at Forest
s/s (cm)
v/d (cm)
Average* 0.286
Research Institute campus, Dehra Dun in 1979. As
reported by Gupta (1982), one clump flowered in
1981 along the Tuli-Wokha road of Nagaland,

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