Dendrocalamus giganteus is one of the biggest and largest bamboo of Bangladesh which is locally known as bhudum bansh. The conventional
propagation methods of bamboos are not economically viable for large scale production due to their scarcity of seeds, low multiplication rate, labor intensive and high cost. In vitro propagation is becoming a promising tool for conserving and mass propagation of different bamboo species. In this study establishment of a reliable and reproducible protocol for the micro propagation of D. giganteus from axenic culture of in vitro germinated seedlings has been reported. Highest 83.33% seeds were germinated on MS (Murashige and Skooge 1962) medium supplemented with 1.0 mg/L BAP (6-benzyl -amino-purine) after 7 days of culture. MS supplemented with different concentrations (0.0, 1.0, 2.0, 3.0 and 4.0 mg/L) of BAP and Kn (6-furfuralaminopurine) at evaluated either singly or in combinations for multiple shoot production. Maximum 16.33 numbers of young shoots per culture were recorded in medium supplemented with MS + 3.0 mg/L BAP + 1.0 mg/L Kn + 4% sugar + 2.75 g/L after 28 days of culture. Rooting ability of the shoots was assessed in half strength MS media supplemented with different concentrations (0.0, 1.0, 2.0, 3.0, and 4.0 mg/L) of IBA (Indole-3-butyric acid). The highest rooting percentage (66.67%) was achieved from the half strength MS medium supplemented with 1.0 mg/L IBA 2 after weeks of culture. The rooted plantlets were successfully hardened in soil under greenhouse and nursery of Silviculture Genetics Division, Bangladesh Forest Research Institute. The survival percentage of tissue culture plantlets in nursery was found to be 90-95% after 60 days of acclimatization. The protocol developed through this study enable to produce large number of D. giganteus bamboo seedlings for mass propagation in a short period of time.