A Half-yearly Peer Reviewed Journal of Bangladesh Forest Research Institute

ISSN - Print: 1021-3279 | Online: -

The in vitro regeneration of Passion fruit (Passiflora edulis L.) was obtained from
cotyledon derived callus and adventitious buds. The callus was initiated in the MS basal
medium supplementedwith different concentrations of plant growth regulators viz, BAP,
KIN, NAA, IAA, and GA3 alone or in combination. Among the different plant growth
regulatorsupplements, 0.2 mg/1 NAA was proved to be more effective in inducing callus
than adventitious bud regeneration. Adventitious bud regeneration was achieved through
organogenesis of callusinMS culturemedium supplementedwithBAP or KIN in combination
withNAA orIAA .The media having 2.0 mg/1BAP+0.2 mg/1 NAA was found to be the best
for adventitious bud regeneration (75%). For shoot regeneration via callus plant growth
regulator cytokinines (BAP & KIN) were supplemented alone orin combination with auxins
(NAA) in MS medium. On the other hand, when BAP or KIN was tried alone, no shoot
regeneration was observed via callus. Among the different combinations of auxins (NAA)
and cytokinines (BAP and KIN) plant growth regulators, 4 mg/1 BAP+0.2 NAA wasfound to
be the bestfor regeneration via callus. Regenerated shoots were rooted in fullstrength MS
medium. IBA (7.0mg/1) effectivelyproduced higher percentage (90%) ofroots and root growth.
Aftersequential hardening, the regenerated plantlets were successfully established in soil
under natural environment.

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