The colonization status of arbuscular mycorrhizal (AM) fungi of Melia azedarach L. seedlings grown from seven different types of pre-sowing treated seeds and the spore population in the rhizosphere soils were studied. The percent root colonization varied significantly and ranged between 14.81-95.0 in different treatments. The superior seedlings showed heavy root colonization as compared to otherseedlings. The intensity of colonization and spore population also varied significantly in different treatments. Spore population was not correlated with the percent root colonization and seedling growth parameters. Four AM genera such Glomus, Acaidospora, Entrophospora and Gigaspora were identified from the rhizosphere soils of the seedlings. The importance of AM fungi has been ensured from this study for the primary establishment of M. azedarach seedlings in the nursery.