A rapid micro-propagation protocol was established for Gynura procumbens (Lour.) Merr., an important medicinal plant for the treatment of
various ailments such as diabetes, hypertension and urinary tract infection. The shoot tips of three months old plants were used as the explants for the initiation of in vitro culture in Murashige and Skoog (MS) medium supplemented with Img/L BAP. Optimization of rapid proliferation of shoots was carried out by culturing the in vitro derived shoots onto MS medium supplemented with different concentrations of BAP and KIN (0.0, 0.5, 1.0,1.5, 2.0 mg/L). Maximum shoot proliferations an average of 21.33 shoots were produced per culture from each shoot in 1.0 mg/L BAP. The effect of different strength of sugar (10, 20, 30, 40, 50 gm/L) and sub culturing on culture medium were observed for optimization of shoot producing. The micro-shoots produced normal roots within two weeks of culture on the basic % MS medium supplemented with 0.5 mg/L IBA. The rooted plantlets of G. procumbens were transferred in soil and kept under green house for hardening with a temperature of 25-29°C and 90% relative humidity for two weeks. About 99% of the plantlets survived after two weeks of transferring into polybag with soil:cowdung (3:1) in the nursery bed. The tissue culture plants showed normal growth and development in poly bag within 6-8 weeks. The regenerated shoots were macro proliferated and produced a large number of new plants in the nursery within a short period of time.