Casuarina equisetifolia seedlingsinoculated with different biofertilizerssuch as Azospirillum, Phosphobacterium, VAM fungi and Frankia and their combinations were planted in farmland. Growth parameters were collected at bimonthly intervals up to 30 months. Biomass was estimated 24 months after planting. Maximum height, girth at breast height (gbh) and total biomass were obtained in the combined application of Azospirillum, Phosphobacterium, VAM and Frankia. The combination of VAM + Frankia among double inoculation, and the combination of Azospirillutn, VAM and Frankia in triple inoculation also proved to be the best treatment in promoting the significant total height, gbh and total biomass production.