A protocol for regeneration of plan tlets from hybrid acacia (Acacia auriculiformis x A. mangium) trees was achieved through tissue culture technique. Effects of MS and B5 culture media supplemented with various concentration of different phytohormones were tested for the induction and proliferation of shoots. The etiolated shoot was cultured and proliferation was found best on MS medium supplemented with BAP (1 mg/1). Proliferation and growth of shoots were further improved through subculture. These shoots produced well developed roots when
cultured on 1/2 MS containing 1.5 mg/1 IBA supplemented with activated charcoal. Ninety per cent regenerated plants survived in the field.