This paper presents the vascular plants (Angiosperms. Gymnosperms and Pteridophyta) of Hazarikhil Wildlife Sanctuary (HWS) in Bangladesh, which is managed by the Chattogram North Forest Division, have been rediscovered. A total of 462 species belonging to 313 genera under 100 families has been documented from the HWS of about 1177.53 ha. Habit analysis of vascular plants that, 184 are trees (40%). 69 shrubs (15%), 148 herbs (32%) and 61 species are climbers (13%) and two epiphytes. Euphorbiaceae is the dominant family represented by 29 taxa, followed by Fabaceae, Rubiaceae and Poaceae (24 taxa each), Asteraceae and Moraceae (16 taxa each), Acanthaceae (15 taxa), Convolvulaceae, Mimosaceae, and Verbenaceae (13 taxa each), Caesalpiniaceae (10 taxa) Lamiaceae, Sterculiaceae and Arecaceae (9 taxa each), Malvaceae and Anacardiaceae (8 taxa each), Amaranthaceae, Apocynaceae, Lauraceae, Araceae Meliaceae. Myrtaceae, Cyperaceae (7 taxa each) and Vitaceae (6 taxa). The Most common species was found the Ficus genera from this sanctuary. The Gymnosperm species of Taxodium distichum (L.) Rich was first recorded in Bangladesh from the study area. Nineteen rare species have been found whereas their plant genetic resources of this sanctuary. The native and tall tree species of the stratum has been described. A list of native trees suitable for reforestation in the HWS has been provided. The species are enumerated with scientific name, family name, bangla name(s), habit and habitat. The HWS is conserved by management plan for execution of National Conservation Strategy.