An in vitro direct regeneration protocol was developed for Phylanthus emblica L. through shoot tip
culture. Shoot tips explants excised from 30 days old aseptically germinated seedlings were inoculated on
Murashige and Skoog (MS) medium supplemented with different concentrations and combinations of BAP, KIN,
NAA and IBA to study in vitro growth responses. MS medium containing 1.0 mg/LBAP was found most suitable
for culture initiation. Although shoot multiplication was achieved on MS medium containing BAP and KJN, the
maximum number of shoot was obtained with 2.0 mg/L BAP+1.0 mg/L KIN. Best rooting response was
observed on media containing half strength MS salts, 0.5 mg/L IBA, 2% sucrose, and 2.8 gm/L gelrite. Plantlets
were hardened initially in culture room conditions and then transferred to mist house. Regenerated plants were
successfully acclimatized and about 90% of plantlets survived under ex vitro conditions. The plant regeneration
system established in this study will facilitate mass propagation of P.emblica in a short period of time.